Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Semenogelin I-derived peptide SgI-52 binds membrane Peroxiredoxin 6 and induces oxidative stress in asthenozoospermic sperm

doi: 10.1186/s12958-025-01516-7

Figure Lengend Snippet: Analysis of the binding between SgI-52 and PRDX 6. A Theoretical analysis of the binding between SgI-52 and single-chain human PRDX 6 (a), and the binding between SgI-52 and double-chain human PRDX 6 (b), utilizing AlphaFold 3 for structural predictions and interaction modeling. Green: SgI-52. Orange: PRDX 6 (PDB:5B6M). B Parameters of the binding interaction between SgI-52 and PRDX 6 from BLI. KD = 2.6 ±1.1 μM (automatically calculated and exported by the BLI instrument's data analysis software using a 1:1 binding model)

Article Snippet: Then, the sperm were incubated with PRDX 6 Monoclonal antibody (67499-1-Ig, Proteintech), anti-SgI-52 Polyclonal antibody for overnight at 4°C, and incubated with CoraLite488-conjugated anti-rabbit secondary antibody and CoraLite594-conjugated anti-mouse secondary antibody for 2 h at 37°C.

Techniques: Binding Assay, Software

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Semenogelin I-derived peptide SgI-52 binds membrane Peroxiredoxin 6 and induces oxidative stress in asthenozoospermic sperm

doi: 10.1186/s12958-025-01516-7

Figure Lengend Snippet: Visualization of PRDX 6 and SgI-52 using immunofluorescence microscopy and immunoelectron microscopy. A Immunocytofluorescence for PRDX 6 and SgI-52. Yellow fluorescence shows SgI-52(green) with PRDX 6(red). Partial co-localization of the proteins is seen in the neck and middle of the sperm. Bar = 10 µm. B Immunogold electron micrographs for sperm. The smaller beads, 5 nm in diameter, represent SgI-52. The larger beads, 12 nm, represent PRDX 6. The signals of PRDX 6 and SgI-52 are distributed on the sperm cell membranes. Note that the gold particles of PRDX 6 are very close to the gold particles of SgI-52

Article Snippet: Then, the sperm were incubated with PRDX 6 Monoclonal antibody (67499-1-Ig, Proteintech), anti-SgI-52 Polyclonal antibody for overnight at 4°C, and incubated with CoraLite488-conjugated anti-rabbit secondary antibody and CoraLite594-conjugated anti-mouse secondary antibody for 2 h at 37°C.

Techniques: Immunofluorescence, Microscopy, Immuno-Electron Microscopy, Fluorescence

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Semenogelin I-derived peptide SgI-52 binds membrane Peroxiredoxin 6 and induces oxidative stress in asthenozoospermic sperm

doi: 10.1186/s12958-025-01516-7

Figure Lengend Snippet: Analysis of PRDX 6 and SgI-52 expression. A Representative images of immunoblotting of ATPA1 and PRDX 6 expression in the normal and AZS sperm. ATP1A1 as a protein loading control. B Seminal plasma (1:25) was immunoblotted (reducing conditions) with anti-SgI-52 antibody as described in “Materials and Methods”. Membranes were finally stained with silver to confirm equal loading in each well; the band presented is that of 33 kDa

Article Snippet: Then, the sperm were incubated with PRDX 6 Monoclonal antibody (67499-1-Ig, Proteintech), anti-SgI-52 Polyclonal antibody for overnight at 4°C, and incubated with CoraLite488-conjugated anti-rabbit secondary antibody and CoraLite594-conjugated anti-mouse secondary antibody for 2 h at 37°C.

Techniques: Expressing, Western Blot, Control, Clinical Proteomics, Staining